Plant Science Colloquium with Prof. Nicolas Rouhier Iron is an essential microelement because numerous metabolic processes and electron transfer reactions rely on heme-and iron-sulfur (Fe-S)-containing proteins. In particular, a high demand on Fe-S clusters/proteins exists in chloroplasts and mitochondria. Photosynthetic organisms possess a specific protein machinery in each organelle for Fe-S cluster biogenesis. This requires a de novo assembly step followed by the transfer of the preformed Fe-S cluster to client proteins using a set of ‘late-acting’ maturation factors. In the latter category, Arabidopsis thaliana and Chlamydomonas reinhardtiipossesses respectively five and three genes encoding NFU proteins which are localized in plastids or in mitochondria. In recent years, we have combined molecular and physiological studies of A. thaliana insertion mutants to the biochemical and spectroscopic characterization of recombinant proteins to delineate the partners and functions of these maturation factors. https://www.inres.uni-bonn.de/en/about/events/plant-science-colloquium-with-prof-nicolas-rouhier https://www.inres.uni-bonn.de/++resource++plone-logo.svg

Iron is an essential microelement because numerous metabolic processes and electron transfer reactions rely on heme-and iron-sulfur (Fe-S)-containing proteins. In particular, a high demand on Fe-S clusters/proteins exists in chloroplasts and mitochondria. Photosynthetic organisms possess a specific protein machinery in each organelle for Fe-S cluster biogenesis. This requires a de novo assembly step followed by the transfer of the preformed Fe-S cluster to client proteins using a set of ‘late-acting’ maturation factors. In the latter category, Arabidopsis thaliana and Chlamydomonas reinhardtiipossesses respectively five and three genes encoding NFU proteins which are localized in plastids or in mitochondria. In recent years, we have combined molecular and physiological studies of A. thaliana insertion mutants to the biochemical and spectroscopic characterization of recombinant proteins to delineate the partners and functions of these maturation factors.